{"id":584689,"date":"2024-11-05T20:40:55","date_gmt":"2024-11-05T20:40:55","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/iso-110632012\/"},"modified":"2024-11-05T20:40:55","modified_gmt":"2024-11-05T20:40:55","slug":"iso-110632012","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/iso\/iso-110632012\/","title":{"rendered":"ISO 11063:2012"},"content":{"rendered":"<p>This International Standard specifies a method for direct extraction of DNA from soil samples to analyse the<\/p>\n<p>global structure and the abundance of soil bacterial communities using PCR-based technologies. This method<\/p>\n<p>is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in<\/p>\n<p>organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.<\/p>\n<p>The direct extraction of DNA from soil samples provides unique insight into the richness and structure of<\/p>\n<p>microbial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecular<\/p>\n<p>approaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promising<\/p>\n<p>domain and can contribute in the near future to the development of routine tools to monitor the microbiota of<\/p>\n<p>soil environments.<\/p>\n<p>Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sieved<\/p>\n<p>thoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as a<\/p>\n<p>result, traces of plant DNA can contaminate the soil DNA extract.<\/p>\n","protected":false},"excerpt":{"rendered":"<p><b>Soil quality \u2014 Method to directly extract DNA from soil samples<\/b><\/p>\n<table style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/iso\/\"><b>ISO<\/b><\/a><\/td>\n<td>2012-02<\/td>\n<td>30<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":584703,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[225,2634],"product_tag":[],"class_list":{"0":"post-584689","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-13-080-30","7":"product_cat-iso","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/584689","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/584703"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=584689"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=584689"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=584689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}