Shopping Cart

No products in the cart.

ASTM-D6974 2009

$40.63

D6974-09 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

Published By Publication Date Number of Pages
ASTM 2009 5
Guaranteed Safe Checkout
Categories: ,

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

ASTM D6974-09

Historical Standard: Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures

ASTM D6974

Scope

1.1 This practice covers a membrane filter (MF) procedure for the detection and enumeration of Heterotrophic bacteria (HPC) and fungi in liquid fuels with kinematic viscosities 24 mm2 · s-1 at ambient temperature.

1.2 This quantitative practice is drawn largely from IP Method 385 and Test Method D 5259.

1.3 This test may be performed either in the field or in the laboratory.

1.4 The ability of individual microbes to form colonies on specific growth media depends on the taxonomy and physiological state of the microbes to be enumerated, the chemistry of the growth medium, and incubation conditions. Consequently, test results should not be interpreted as absolute values. Rather they should be used as part of a diagnostic or condition monitoring effort that includes other test parameters, in accordance with Guide D 6469.

1.5 This practice offers alternative options for delivering fuel sample microbes to the filter membrane, volumes or dilutions filtered, growth media used to cultivate fuel-borne microbes, and incubation temperatures. This flexibility is offered to facilitate diagnostic efforts. When this practice is used as part of a condition monitoring program, a single procedure should be used consistently.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

bacteria; biodegradation; biodeterioration; colony forming unit; enumeration; fuel microbiology; fungi; membrane filter technique; viable count; Bacteria/bacterial control–pretroleum products; Biodegradation; Biodeterioration; Colony forming units`; Fuel microbiology; Fungal influence/resistance; Membrane filters (MF); Viable bacteria

ICS Code

ICS Number Code 75.160.20 (Liquid fuels)

DOI: 10.1520/D6974-09

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
Terminology
2 Summary of Practice
Significance and Use
Interferences
Apparatus
3 Reagents and Materials
Procedure
5 Report
Precision and Bias
Keywords
ASTM-D6974 2009
$40.63