ASTM-E2719:2009 Edition

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E2719-09 Standard Guide for Fluorescence—Instrument Calibration and Qualification

Published By	Publication Date	Number of Pages
ASTM	2009	17

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Categories: 71.040.50 - Physicochemical methods of analysis, ASTM

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Description

ASTM E2719-09

Historical Standard: Standard Guide for Fluorescence—Instrument Calibration and Qualification

ASTM E2719

Scope

1.1 This guide (1) lists the available materials and methods for each type of calibration or correction for fluorescence instruments (spectral emission correction, wavelength accuracy, and so forth) with a general description, the level of quality, precision and accuracy attainable, limitations, and useful references given for each entry.

1.2 The listed materials and methods are intended for the qualification of fluorometers as part of complying with regulatory and other quality assurance/quality control (QA/QC) requirements.

1.3 Precision and accuracy or uncertainty are given at a 1 σ confidence level and are approximated in cases where these values have not been well established.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

anisotropy; calibration; fluorescence; fluorometer; lifetime; limit of detection; luminescence; qualification; quantum yield; reference materials; spectral correction; spectrometer; spectroscopy; validation; verification; wavelength

ICS Code

ICS Number Code 71.040.50 (Physicochemical methods of analysis)

DOI: 10.1520/E2719-09

PDF Catalog

PDF Pages	PDF Title
1	Scope Referenced Documents Terminology
3	Significance and Use General Guidelines TABLE 1
4	Wavelength Accuracy TABLE 2
5	FIG. 1 FIG. 2
6	FIG. 3 FIG. 4
7	FIG. 5 FIG. 6
8	Spectral Slit Width Accuracy Linearity of the Detection System FIG. 7
9	Spectral Correction of Detection System Responsivity FIG. 8 TABLE 3
10	Spectral Correction of Excitation Beam Intensity FIG. 9 TABLE 4
11	FIG. 10
12	Calibration Curves for Concentration
13	Day-to-Day and Instrument-to-Instrument Intensity Limit of Detection and Sensitivity ( ) (Test Method ) Lifetimes ( , )
14	Fluorescence Quantum Yield ( )
15	Fluorescence Anisotropy ( ) Keywords REFERENCES

Additional information

Standard Title	E2719-09 Standard Guide for Fluorescence—Instrument Calibration and Qualification
Published Code	ASTM
Publication Date	2009
Pages Count	17
ICS Codes	71.040.50 - Physicochemical methods of analysis