BS EN 15634-4:2023

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Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

Published By	Publication Date	Number of Pages
BSI	2023	20

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Description

This document specifies a method for the detection of peanut (Arachis hypogaea) in chocolate. Real-time PCR (Polymerase Chain Reaction) detection of peanut is based on an 86 bp (base pair) sequence from the Ara h 2 gene of peanut.

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PDF Pages	PDF Title
2	undefined
7	1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents 5.1 General 5.2 Extraction reagents
8	5.3 DNA purification by means of solid phase extraction 5.4 Real-time PCR reagents
9	6 Apparatus and equipment 6.1 General 6.2 DNA extraction 6.3 PCR
10	7 Procedure 7.1 General 7.2 Sample preparation 7.3 Preparation of extracts 7.3.1 DNA extraction with CTAB and DNA purification
11	7.3.2 Quantification of DNA concentration and purity/quality check 7.4 Real-time PCR set-up 7.4.1 Reaction mix for real-time PCR
12	7.4.2 Positive control for DNA targets 7.4.3 Negative control for DNA targets
13	7.4.4 PCR inhibition control 7.4.5 Amplification reagent control 7.4.6 Extraction blank control 7.4.7 Positive extraction control 7.4.8 Temperature/time programme (real-time PCR) 7.4.9 Accept/Reject criteria
14	7.4.10 Identification 8 Validation 8.1 General 8.2 Specificity 8.3 Ring trial validation study 8.3.1 Setup of the ring trial study
15	8.3.2 Ring trial validation results 8.3.2.1 Ring trial validation data
16	8.3.2.2 Robustness of the method 8.3.2.3 Limit of detection 9 Test report

Additional information

Status	Definitive
Pages	20
Publication Date	2023-05-16
ISBN	978 0 539 18721 2
Standard Number	BS EN 15634-4:2023
Title	Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
Identical National Standard Of	EN 15634-4
Replaces	PD CEN/TS 15634-4:2016
Descriptors	Food products, Chocolate, Determination of content, Deoxyribonucleic acid, Peanuts
Publisher	BSI
Committee	AW/275
ICS Codes	67.190 - Chocolate

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BS EN 15634-4:2023

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