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BS EN 15786:2021 – TC:2022 Edition

BS EN 15786:2021 – TC:2022 Edition

$186.33

Tracked Changes. Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Pediococcus spp. used as feed additive

Published By Publication Date Number of Pages
BSI 2022 54
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This document specifies general rules for the enumeration of pediococci in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain pediococci as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see A.2). The document is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) 767/2009) [3]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing about 1011 CFU/kg; c) Compound feeds, meal or pellets which contain about 109 CFU/kg.

PDF Catalog

PDF Pages PDF Title
32 undefined
38 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
39 5 Diluents and culture media
5.1 Diluents
40 5.2 Culture media
5.2.1 General
5.2.2 Composition
41 5.2.3 Preparation
42 6 Apparatus
43 7 Sampling
8 Preparation of test sample
44 9 Procedure
9.1 Samples with a single or multi-component added microflora
9.2 Preparation of poured agar plates for spread plate method
9.3 Preparation of MRS agar for pour plate method
9.4 Preparation of the initial suspension and decimal dilutions
45 9.5 Inoculation and incubation of plates
9.5.1 General
46 9.5.2 Spread plate method
9.5.3 Pour plate method
9.6 Enumeration of colonies
9.7 Confirmation
47 10 Expression of results
48 11 Precision
11.1 General
11.2 Interlaboratory study
11.3 Repeatability
11.4 Reproducibility
12 Test report
49 Annex A (informative) Notes on the procedure
A.1 General
A.2 Critical copper concentration
50 Annex B (informative)Results of the interlaboratory study

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BS EN 15786:2021 - TC
$186.33