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BS EN 15789:2021

BS EN 15789:2021

$102.76

Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Saccharomyces cerevisiae used as feed additive

Published By Publication Date Number of Pages
BSI 2021 20
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This document specifies general rules for the enumeration of Saccharomyces cerevisiae in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain Saccharomyces cerevisiae as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see Annex A). The document is not applicable to mineral feeds, which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) 767/2009) [3]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing about 1011 CFU/kg; c) Compound feeds, meal or pellets which contain about 109 CFU/kg.

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Principle
5 Diluents and culture medium
5.1 Diluents
9 5.2 Culture medium
10 6 Apparatus
11 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Preparation of poured agar plates for spread plate method
9.2 Preparation of YGC agar for pour plate method
9.3 Preparation of the initial suspension and decimal dilutions
13 9.4 Inoculation and incubation of plates
9.4.1 General
9.4.2 Spread plate method
9.4.3 Pour plate method
14 9.5 Enumeration of colonies
9.6 Confirmation
10 Expression of results
15 11 Precision
11.1 General
11.2 Interlaboratory study
11.3 Repeatability
11.4 Reproducibility
12 Test report
16 Annex A (informative) Critical copper concentrations
17 Annex B (informative) Results of the interlaboratory study

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BS EN 15789:2021
$102.76