BS EN ISO 10927:2018
$102.76
Plastics. Determination of the molecular mass and molecular mass distribution of polymer species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)
| Published By | Publication Date | Number of Pages |
| BSI | 2018 | 22 |
This document specifies a general method for determining the average molecular mass and molecular mass distribution of polymers (see Reference [1]) from 2 000 g ⋅ mol−1 to 20 000 g ⋅ mol−1 by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
The average molecular masses and molecular mass distributions are calculated from a calibration curve constructed using synthetic-polymer and/or biopolymer standards. This method is therefore classified as a relative method.
The method is not applicable to polyolefins or to polymers with a polydispersity >1,2.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 4 | European foreword |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Principle 5 Reagents 5.1 Matrices 5.2 Solvents |
| 11 | 5.3 Salts 5.4 Molecular mass standards 6 Apparatus 6.1 General 6.2 Sample introduction chamber/target |
| 12 | 6.3 Laser source 6.4 Flight tube 6.5 Detector 6.6 Data recording |
| 13 | 6.7 Data handling 7 Procedure 7.1 General 7.2 Sample preparation 7.2.1 General 7.2.2 Preparation of polymer/matrix/salt solutions 7.2.3 Deposition of the sample on the sample plate (target) |
| 14 | 7.2.4 Preparation and spotting of biopolymer/matrix solutions 7.3 Instrument settings |
| 15 | 7.4 Recording spectra 8 Data acquisition and processing 8.1 General |
| 16 | 8.2 Calibration 8.2.1 General 8.2.2 Calibration of mass axis using synthetic-polymer standards 8.2.3 Calibration of mass axis using biopolymer standards 8.2.4 Self-calibration method 8.3 Generation of calibration curve 8.4 Signal intensity axis calibration |
| 17 | 9 Expression of results 9.1 Calculation of molecular mass distribution 9.2 Calculation of the average molecular masses 10 Precision 11 Test report |
| 19 | Annex A (normative) Calibrants |
| 20 | Annex B (informative) Precision data |
| 21 | Bibliography |
