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BS EN ISO 11268-2:2023

BS EN ISO 11268-2:2023

$189.07

Soil quality. Effects of pollutants on earthworms – Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species

Published By Publication Date Number of Pages
BSI 2023 48
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This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei. Technical information is also provided on how to use Eisenia fetida/andrei for testing chemicals under tropical conditions (see Annex A). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C). This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C. This method does not take into account the persistence of the substance during the test.

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
10 3 Terms and definitions
11 4 Principle
12 5 Reagents and material
14 6 Apparatus
7 Procedure
7.1 Experimental design
7.1.1 General
15 7.1.2 Preliminary test
7.1.3 Definitive test
7.2 Preparation of test mixtures
7.2.1 Testing of contaminated soil
16 7.2.2 Testing substances added to the control soil
7.2.3 Preparation of control container
17 7.3 Addition of the earthworms
7.4 Test conditions and measurements
7.5 Reference substance
18 8 Calculation and expression of results
8.1 Calculation
8.2 Expression of results
9 Validity of the test
10 Statistical analysis
10.1 General
10.2 Single-concentration tests
19 10.3 Multi-concentration tests
10.3.1 Preliminary test
10.3.2 Definitive test
20 11 Test report
21 Annex A (informative) Determination of the chronic toxicity of chemicals on Eisenia fetida/Eisenia andrei under tropical conditions
23 Annex B (informative) Culturing and testing procedures for assessing the effects of contaminants on juvenile production using the earthworm, Dendrodilus rubidus
27 Annex C (informative) Culturing and testing procedures for assessing the effects of contaminants on adult survival and cocoon production and hatchability using the earthworm, Aporrectodea caliginosa s.s.
31 Annex D (informative) Culturing of Eisenia fetida and Eisenia andrei
32 Annex E (normative) Determination of water holding capacity
33 Annex F (informative) Techniques for counting juvenile worms hatched from cocoons
34 Annex G (informative) Experience with performance criteria of the test
41 Bibliography

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BS EN ISO 11268-2:2023
$189.07