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BS EN ISO 18187:2024

BS EN ISO 18187:2024

$167.15

Soil quality. Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis

Published By Publication Date Number of Pages
BSI 2024 36
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This document specifies a rapid method for assessing solid samples in an aerobic suspension, by determining the inhibition of dehydrogenase activity of Arthrobacter globiformis using the redox dye resazurin. It is applicable for assessing the effect of water-soluble and solid matter bounded non-volatile contaminants in natural samples, such as soils and waste materials. Although not the main purpose, the contact test can additionally be used for testing the effect of chemicals, as described in the Annex C. The test yields a result within 6 h and can therefore be used for screening potentially contaminated test material.

PDF Catalog

PDF Pages PDF Title
2 undefined
12 5.1 Test organisms
5.2 Control substrates
5.2.1 General
5.2.2 Control for soils
13 5.2.3 Control for waste material
5.3 Test substrates
14 5.4 Reagents
17 7.1 Preparation of dilutions
7.2 Reference substances and positive control preparation
18 7.3 Contact test procedure
7.3.1 General
7.3.2 Aeration
19 7.3.3 Deactivation
7.3.4 Preparation of the inoculum
7.3.5 Incubation and fluorescence measurement
7.4 Interferences
20 8.1 Calculation
8.1.1 Relative fluorescence
8.1.2 Determining the percentage of inhibition
8.2 Expression of results

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BS EN ISO 18187:2024
$167.15