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BS EN ISO 19040-1:2022

BS EN ISO 19040-1:2022

$198.66

Water quality. Determination of the estrogenic potential of water and waste water – Yeast estrogen screen (Saccharomyces cerevisiae)

Published By Publication Date Number of Pages
BSI 2022 60
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PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
7 Foreword
9 1 Scope
2 Normative references
10 3 Terms and definitions
12 4 Principle
5 Interferences
13 6 Apparatus and materials
14 7 Reagents, media and test strain
18 8 Sampling and samples
8.1 General
8.2 Bottles and material for sampling
8.3 Bottles and material pre-cleaning
8.4 Sampling procedure
19 8.5 Transport of samples
8.6 Pretreatment of samples
8.7 Storage of samples
20 9 Procedure
9.1 Preparation of cryo-cultures for long-term storage
9.2 Overnight culture
9.3 Test set up for aqueous samples
9.3.1 Preparation
9.3.2 Preparation of the reference dilution series
21 9.3.3 Negative control
22 9.3.4 Blank replicate
9.3.5 Sample dilution
9.3.6 Field blank
9.3.7 Plate setup
9.3.8 Inoculation of the test plate
23 9.4 Measurement
9.4.1 Measurement of the cell density
24 9.4.2 Measurement of the reporter gene activity
9.5 Calculation of the corrected absorbance and the reporter gene induction
25 9.6 Calculation of the relative growth
9.7 Estimation of the EC50 of the reference compound by linear interpolation
10 Validity criteria
26 11 ā€‹Assessment criteria
12 Test report
27 Annex A (normative) Strain selection
28 Annex B (informative) Plate set up
29 Annex C (informative) Scheme of test principle
30 Annex D (informative) Test set up for chemicals and extracts
34 Annex E (informative) Preparation of dilution series
35 Annex F (informative) Performance data
48 Annex G (informative) Use of other yeast strains based on Saccharomyces cerevisiae
51 Annex H (informative) Statistical assessment
53 Annex I (informative) Calculation of 17Ī²-estradiol equivalents
56 Annex J (informative) Measurement of the lowest ineffective dilution (LID) of a waste water ā€” A simplified evaluation for testing of waste water
58 Bibliography

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BS EN ISO 19040-1:2022
$198.66