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BS EN ISO 19040-3:2022

BS EN ISO 19040-3:2022

$189.07

Water quality. Determination of the estrogenic potential of water and waste water – In vitro human cell-based reporter gene assay

Published By Publication Date Number of Pages
BSI 2022 50
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PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
7 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
12 4 Interferences
5 Principle
6 Apparatus and materials
13 7 Reagents, cells and media
17 8 Sampling and samples
8.1 General
8.2 Bottles and material for sampling
8.3 Bottles and material pre-cleaning
8.4 Sampling procedure
8.5 Transport of samples
18 8.6 Pretreatment of sample
8.7 Storage of samples
9 Procedure
9.1 Cell culture maintenance
9.1.1 Freezing cells
9.1.2 Starting a new cell culture
19 9.1.3 Culturing cells
9.2 Human cell reporter gene assay test procedure
9.2.1 Seeding the cells (day 1)
9.2.2 Preparation of the E2-reference (day 2)
20 9.2.3 Preparation of the sample dilutions (day 2)
9.2.4 Field blank
9.2.5 Exposing the cells (day 2)
21 9.2.6 Harvesting the cells (day 3)
9.2.7 Measurement of luminescence (day 3)
9.3 Data analysis
9.3.1 Calculation of the reporter gene induction
22 9.3.2 Calculation of the percentage of maximum response
9.3.3 Calculation of the dose-response curve
10 Validity criteria
10.1 Validity criteria for the assay
23 10.2 Validity criteria for samples
11 Assessment criteria
12 Test report
24 Annex A (informative) Settings of the luminometer
25 Annex B (informative) Plate setup
26 Annex C (informative) Bioassay characteristics and details
28 Annex D (informative) Test set up for chemicals and extracts
30 Annex E (informative) Preparation of dilution series
31 Annex F (informative) Performance data
41 Annex G (informative) Statistical assessment
42 Annex H (informative) Calculation of 17β-estradiol equivalents
44 Annex I (informative) Measurement of the lowest ineffective dilution (LID) of waste water — A simplified evaluation for testing of waste water
47 Bibliography

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BS EN ISO 19040-3:2022
$189.07