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BS EN ISO 20136:2020

BS EN ISO 20136:2020

$167.15

Leather. Determination of degradability by micro-organisms

Published By Publication Date Number of Pages
BSI 2020 34
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This document specifies a test method to determine the degree and rate of aerobic biodegradation of hides and skins of different animal origin, whether they are tanned or not, through the indirect determination of CO2 produced by the degradation of collagen.

The test material is exposed to an inoculum (activated sludge from tannery wastewater) in an aqueous medium. If there is not a tannery nearby then urban wastewater can be used as the inoculum.

The conditions established in this document correspond to optimum laboratory conditions to achieve the maximum level of biodegradation. However, they might not necessarily correspond to the optimum conditions or maximum level of biodegradation in the natural medium.

In general, the experimental procedure covers the determination of the degradation degree and rate of the material under controlled conditions, which allows the analysis of the evolved carbon dioxide produced throughout the test. For this purpose, the testing equipment complies with strict requirements with regard to flow, temperature and agitation control.

This method applies to the following materials:

  1. natural polymers of animal stroma (animal tissue/skins);

  2. animal hides and skins tanned (leather) using organic or inorganic tanning agents;

  3. leathers that, under testing conditions, do not inhibit the activity of microorganisms present in the inoculum.

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Symbols and abbreviated terms
5 Principle
5.1 General
5.2 ​Assessment of biodegradation by manual titration; method A
11 5.3 ​Assessment of biodegradation by infrared (IR) detection; method B
6 Chemicals
12 7 Apparatus and materials
15 8 Procedure
8.1 Collection and preparation of the inoculum
8.2 Preparation of the test material and reference material
8.3 Test conditions and incubation period
8.4 Termination of the test
16 9 Quantification
9.1 ​Assessment of biodegradation by manual titration (method A)
9.1.1 Determination of the organic carbon content
9.1.2 Determination of the amount of CO2 produced
9.1.3 Correcting for normality of HCl
17 9.1.4 Percentage of biodegradation from CO2 evolved
9.2 ​Assessment of biodegradation by IR (method B)
9.2.1 Determination of the organic carbon content
18 9.2.2 Determination of the amount of CO2 produced
9.2.3 Percentage of biodegradation from CO2 data
23 10 Expression of results
11 Validity of results
12 Test report
24 Annex A (informative) Determination of the degree and rate of degradation of the material
27 Annex B (informative) Quantitative determination of leather biodegradation
31 Annex C (informative) Comparative biodegradability using different waste waters
32 Bibliography

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BS EN ISO 20136:2020
$167.15