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BS EN ISO 21149:2017

BS EN ISO 21149:2017

$167.15

Cosmetics. Microbiology. Enumeration and detection of aerobic mesophilic bacteria

Published By Publication Date Number of Pages
BSI 2017 36
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This document gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics

  • by counting the colonies on agar medium after aerobic incubation, or

  • by checking the absence of bacterial growth after enrichment.

Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.

If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
Endorsement notice
7 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 General
4.2 Plate count
4.3 Membrane filtration
11 4.4 Detection of bacteria by enrichment
5 Diluents, neutralizers and culture media
5.1 General
5.2 Neutralizing diluents and diluents
12 5.3 Diluent for the bacterial suspension (tryptone sodium chloride solution)
5.4 Culture media
15 6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension
9.2.1 General
16 9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.3 Counting methods
9.3.1 Dilutions for counting methods
9.3.2 Plate-count methods
17 9.4 Enrichment
9.4.1 General
9.4.2 Incubation of the sample
10 Counting of colonies (plate counts and membrane filtration methods)
11 Detection of growth (enrichment method)
18 12 Expression of results
12.1 Method of calculation for plate count
19 12.2 Interpretation
12.3 Examples
21 12.4 Detection after enrichment
13 Neutralization of the antimicrobial properties of the product
13.1 General
22 13.2 Preparation of inoculum
13.3 Suitability of counting methods
13.3.1 Principle
13.3.2 Suitability test of the pour-plate method
13.3.3 Suitability of the surface spread method
13.3.4 Suitability of the membrane filtration method
23 13.4 Suitability of the detection method by enrichment
13.4.1 Procedure
13.4.2 Interpretation of results
13.5 Interpretation of suitability test results
24 14 Test report
25 Annex A (informative) Other neutralizing diluents
27 Annex B (informative) Other diluents
28 Annex C (informative) Other culture media
31 Annex D (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
32 Bibliography

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BS EN ISO 21149:2017
$167.15