BS EN ISO 23753-2:2019+A1:2020:2021 Edition
$102.76
Soil quality Determination of dehydrogenases activity in soils – Method using iodotetrazolium chloride (INT)
| Published By | Publication Date | Number of Pages |
| BSI | 2021 | 18 |
This document specifies a method for determining activity of dehydrogenases in soil, using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)[1]-[5]. As the INT reduction is less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753‑1.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 4 | European foreword Anchor 4 Foreword to amendment A1 |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Limitations |
| 10 | 6 Reagents and materials 6.1 Soil 6.5 Analytical grade acetone. 6.6 INTF stock solution. 7 Apparatus 7.1 Visible light spectrophotometer. 7.2 pH-meter. |
| 11 | 7.6 Ultrasonic probe. 7.7 Orbital tube shaker. 8 Procedure 8.1 Establishment of standard curve 8.2 Sampling |
| 12 | 9 Calculation 10 Validity criteria 10.1 Standard curve |
| 13 | 10.2 Samples 11 Test report |
| 14 | Annex A (informative) Results of modified parameters |
| 16 | Bibliography |
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