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BS EN ISO 8655-8:2022

BS EN ISO 8655-8:2022

$142.49

Piston-operated volumetric apparatus – Photometric reference measurement procedure for the determination of volume

Published By Publication Date Number of Pages
BSI 2022 28
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This document specifies the photometric reference measurement procedure for the determination of volume of piston-operated volumetric apparatus (POVA). The procedure is applicable to complete systems comprising the basic apparatus with a maximum nominal volume of 5 000 Āµl and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery (Ex).

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 General requirements
5 Test equipment
5.1 General
5.2 Spectrophotometer
5.3 Cuvette and mixer
11 5.4 Measuring devices
5.5 Equipment used for solution preparation
5.6 Balances
12 5.7 Density meter
5.8 pH meter
6 Reagents
6.1 General requirements
6.2 Water
6.3 Buffer solution
6.4 Copper(II) chloride solution
6.5 Ponceau S solutions
13 6.6 Calibrator solutions
14 6.7 Stability of solutions
6.7.1 General
6.7.2 Preservatives
6.7.3 Light sensitivity
6.7.4 Storage temperature
7 Test conditions
7.1 General
7.2 Test room
15 7.3 Evaporation
8 Procedure
8.1 General
8.1.1 Summary
8.1.2 Test conditions
8.1.3 Test volume
8.1.4 Number of measurements per volume to be tested
16 8.2 System calibration
8.2.1 General
8.2.2 System calibration procedure
8.2.3 Previous calibration
8.3 Photometric procedure
8.3.1 Preparation of cuvettes
8.3.2 Zero of the spectrophotometer
8.3.3 Starting absorbances
17 8.3.4 Dispensing of test liquids
8.3.5 Absorbance of the chromophore mixture
8.3.6 Calculation of the delivered test volume
8.4 Preparation
8.5 Single-channel air displacement pipettes (in accordance with ISO 8655ā€‘2)
8.5.1 General
18 8.5.2 Test cycle
19 8.6 Multi-channel pipettes (in accordance with ISO 8655ā€‘2)
8.7 Positive displacement pipettes (in accordance with ISO 8655ā€‘2)
8.8 Burettes (in accordance with ISO 8655ā€‘3)
20 8.9 Dispensers (in accordance with ISO 8655ā€‘5)
8.10 Syringes (in accordance with ISO 8655ā€‘9)
8.10.1 General
8.10.2 Test cycle
21 9 Evaluation
9.1 Calculation of volume
9.1.1 Calibration constant
9.1.2 Volume of test liquid
9.1.3 Temperature correction
22 9.1.4 Mean volume
9.2 Systematic error of measurement
9.3 Random error of measurement
23 9.4 Uncertainty of measurement
10 Reporting of results
25 Annex A (normative) Calculation of volumes from balance readings
26 Bibliography

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BS EN ISO 8655-8:2022
$142.49