BS ISO 18337:2015
$102.76
Surface chemical analysis. Surface characterization. Measurement of the lateral resolution of a confocal fluorescence microscope
| Published By | Publication Date | Number of Pages |
| BSI | 2015 | 22 |
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Terms and definitions 3 Symbols and abbreviated terms 4 General 4.1 Background information |
| 10 | 4.2 Types of CFM operation 4.2.1 General 4.2.2 Stage scanning CFM 4.2.3 Laser scanning CFM 4.2.4 Spinning disk CFM |
| 11 | 4.3 Parameters that affect the lateral resolution of a CFM 4.3.1 General 4.3.2 Objective lens 4.3.3 Detection pinhole size and focal length of the tube lens 4.3.4 Collimation and purity of the laser illumination beam 4.3.5 Polarization of the laser illumination 4.3.6 Excitation and emission wavelengths 4.3.7 Image contrast 5 Measuring the lateral resolution by imaging a small object 5.1 Background information |
| 12 | 5.2 Selection of the sample and sample requirements 5.3 Setting the parameters prior to operating the instrument |
| 13 | 5.4 Data collection and analysis 5.4.1 Selecting a proper spot 5.4.2 Extracting a line profile using band-average process |
| 14 | 5.5 Recording the data |
| 15 | Annex A (informative) Sample preparation and example of data and analysis |
| 18 | Bibliography |
