BSI 23/30459164 DC:2023 Edition

$13.70

BS EN 1657 Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements (phase 2, step 1)

Published By	Publication Date	Number of Pages
BSI	2023	46

Guaranteed Safe Checkout

Categories: 71.100.35 - Chemicals for industrial and domestic disinfection purposes, BSI

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

Description

PDF Catalog

PDF Pages	PDF Title
7	1 Scope 2 Normative references 3 Terms and definitions 4 Requirements
9	5 Test method 5.1 Principle 5.2 Materials and reagents 5.2.1 Test organisms
10	5.2.2 Culture media and reagents 5.2.2.1 General 5.2.2.2 Water 5.2.2.3 Malt extract agar (MEA)
11	5.2.2.4 Diluent 5.2.2.5 Neutralizer 5.2.2.6 Rinsing liquid (for membrane filtration) 5.2.2.7 Hard water for dilution of products
12	5.2.2.8 Interfering substance 5.3 Apparatus and glassware 5.3.1 General
13	5.3.2 Usual microbiological laboratory equipment) and, in particular, the following 5.3.2.1 Apparatus for sterilization
14	5.4 Preparation of test organism suspensions and product test solutions 5.4.1 Test organism suspensions (test and validation suspension) 5.4.1.1 General 5.4.1.2 Preservation and stock cultures of test organisms 5.4.1.3 Working culture of test organisms
15	5.4.1.4 Test suspension (“N”)
18	5.4.1.5 Validation suspension (“Nv”) 5.4.1.6 Incubation and counting of the test and the validation suspensions 5.4.2 Product test solutions
19	5.5 Procedure for assessing the fungicidal or yeasticidal activity of the product 5.5.1 General 5.5.1.1 Experimental conditions (obligatory and additional) 5.5.1.2 Choice of test method (dilution-neutralization or membrane filtration)
20	5.5.1.3 General instructions for validation and control procedures 5.5.1.4 Equilibration of temperature 5.5.1.5 Precautions for manipulation of test organisms 5.5.2 Dilution-neutralization method ) 5.5.2.1 General 5.5.2.2 Test “Na” – determination of fungicidal or yeasticidal concentrations
21	5.5.2.3 Experimental conditions control “A” – validation of the selected experimental conditions and/or verification of the absence of any lethal effect in the test conditions 5.5.2.4 Neutralizer control “B” – verification of the absence of toxicity of the neutralizer
22	5.5.2.5 Method validation “C” – dilution-neutralization validation 5.5.2.6 Incubation and counting of the test mixture and the control and validation mixtures 5.5.3 Membrane filtration method) 5.5.3.1 General
23	5.5.3.2 Test “Na” – determination of the fungicidal or yeasticidal concentrations 5.5.3.3 Experimental conditions control “A” – validation of the selected experimental conditions and/or verification of the absence of any lethal effect in the test conditions 5.5.3.4 Filtration control “B” – validation of the filtration procedure
24	5.5.3.5 Method validation “C” – validation of the membrane filtration method or counting of the fungi on the membranes which have previously been in contact with the mixture of product and interfering substance 5.5.3.6 Incubation and counting of the test mixture and the control and validation mixtures 5.6 Experimental data and calculation 5.6.1 Explanation of terms and abbreviations 5.6.1.1 Overview of the different suspensions and test mixtures
25	5.6.1.2 Vc values 5.6.2 Calculation 5.6.2.1 General 5.6.2.2 Determination of Vc values
26	5.6.2.3 Calculation of N and N0 5.6.2.4 Calculation of Na
27	5.6.2.5 Calculation of Nv and Nv0
28	5.6.2.6 Calculation of A, B and C 5.7 Verification of methodology 5.7.1 General 5.7.2 Control of weighted mean counts 5.7.3 Basic limits
29	5.7.4 Additional limits for Aspergillus brasiliensis 5.8 Expression of results and precision 5.8.1 Reduction 5.8.2 Control of active and non-active product test solution (5.4.2) 5.8.3 Limiting test organism and fungicidal/yeasticidal concentration 5.8.3.1 Fungicidal concentration 5.8.3.2 Yeasticidal concentration 5.8.4 Precision, replicates 5.9 Interpretation of results – conclusion 5.9.1 General
30	5.9.2 Fungicidal activity for general purposes 5.9.3 Fungicidal activity for specific purposes 5.9.4 Yeasticidal activity for general purposes 5.9.5 Yeasticidal activity for specific purposes 5.9.6 Yeasticidal activity for teat disinfectants 5.10 Test report
32	Annex A (informative)Referenced strains in national collections
33	Annex B (informative)Suitable neutralizers and rinsing liquids
35	Annex C (informative)Graphical representation of test procedures Dilution-neutralization method
37	C.2 Membrane filtration method
39	Annex D (informative)Example of a typical test report
43	Annex E (informative)Precision of the test result

Additional information

Status	Definitive
Pages	46
Publication Date	2023-05-10
Standard Number	23/30459164 DC
Title	BS EN 1657 Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements (phase 2, step 1)
Identical National Standard Of	EN 1657
Descriptors	Fungicides, Suspensions (chemical), Disinfectant tests, Microbiological-resistance tests, Quantitative analysis, Fungal-resistance tests, Veterinary science, Disinfectants, Microbiological analysis, Antiseptics
Publisher	BSI
Committee	CH/216
ICS Codes	71.100.35 - Chemicals for industrial and domestic disinfection purposes

Preview PDF


PDF Format	Searchable	Printable	Preview Now

BSI 23/30459164 DC:2023 Edition

PDF Catalog

Related products