BSI 23/30461320 DC:2023 Edition
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BS EN ISO 10253. Water quality. Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum
Published By | Publication Date | Number of Pages |
BSI | 2023 | 33 |
PDF Catalog
PDF Pages | PDF Title |
---|---|
6 | Foreword |
7 | 1 Scope 2 Normative references 3 Terms and definitions |
8 | 4 Principle |
9 | 5 Materials 5.1 Test organisms |
10 | 5.2 Reagents 5.2.1 Water 5.2.2 Synthetic Sea water 5.2.3 Nutrients |
11 | 6 Apparatus |
12 | 7 Procedure 7.1 Preparation of growth medium 7.2 Preparation of pre-culture and inoculum 7.3 Choice of test concentrations |
13 | 7.4 Preparation of test substance stock solutions 7.5 Preparation of test and control batches 7.6 Incubation |
14 | 7.7 Measurements 8 Validity criteria 9 Interpretation of data 9.1 Plotting growth curves |
15 | 9.2 Calculation of percentage inhibition 9.3 Determination of EC(r)x 10 Expression of results |
16 | 11 Interpretation of results 12 Test report |
17 | Annex€A (informative) Preparation of dilution series of mixtures in sea water(effluents or elutriates) |
18 | Annex€B (informative) Test procedure starting from stored algal inocula, and with direct measurement of algal growth in spectrophotometric cells |
24 | Annex€C (informative) Performance data |
25 | Annex€D (informative) Marine algal growth inhibition test with Phaeodactylum tricornutum applied in 24-well-microwell plates |
33 | Bibliography |