BSI PD ISO/TS 23459:2021

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Nanotechnologies. Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism

Published By	Publication Date	Number of Pages
BSI	2021	36

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This document specifies measurement protocols and test conditions to determine alterations to protein secondary structure induced by their interaction with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy.

This document does not apply to the characterization of conformational changes of disordered proteins.

PDF Catalog

PDF Pages	PDF Title
2	undefined
6	Foreword
7	Introduction
9	1 Scope 2 Normative references 3 Terms and definitions
10	4 Abbreviated terms 5 Nanomaterial protein interactions
11	6 Sample preparation 6.1 General 6.2 Desired properties of the UV-CD quartz cell 6.3 Preparation of protein solution
12	6.4 Instrumental setting condition 6.5 Recording UV-CD spectra procedure 6.5.1 General 6.5.2 Buffer 6.5.3 Protein sample
13	6.5.4 Stability of NP suspension in the protein solution 6.6 Preparation of protein-NPs conjugated suspension 6.7 UV-CD spectra measurement
14	6.8 Calculation of molar ellipticity 6.9 Data analysis
15	7 Test report
16	Annex A (informative) Typical UV-CD spectra of proteins
17	Annex B (informative) Literature survey on structural changes of NOAA and proteins
23	Annex C (informative) Description of buffers that can be used for protein solubility
27	Annex D (informative) Unit conversions in CD measurements
28	Annex E (informative) Calculating the concentration range of the sample
29	Annex F (informative) Methods for estimation of secondary structures of protein
30	Annex G (informative) Typical data of UV-CD used for estimation of secondary structures of protein
31	Bibliography