{"id":14636,"date":"2024-10-16T21:40:14","date_gmt":"2024-10-16T21:40:14","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/astm-e2719\/"},"modified":"2024-10-24T12:03:26","modified_gmt":"2024-10-24T12:03:26","slug":"astm-e2719","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/astm\/astm-e2719\/","title":{"rendered":"ASTM-E2719"},"content":{"rendered":"

ASTM E2719-09<\/h3>\n

Historical Standard: Standard Guide for Fluorescence\u2014<\/span>Instrument Calibration and Qualification<\/h4>\n
\n

ASTM E2719<\/h4>\n

Scope<\/strong><\/p>\n

1.1 This guide (1)<\/span> lists the available materials and methods for each type of calibration or correction for fluorescence instruments (spectral emission correction, wavelength accuracy, and so forth) with a general description, the level of quality, precision and accuracy attainable, limitations, and useful references given for each entry.<\/p>\n

1.2 The listed materials and methods are intended for the qualification of fluorometers as part of complying with regulatory and other quality assurance\/quality control (QA\/QC) requirements.<\/p>\n

1.3 Precision and accuracy or uncertainty are given at a 1 \u03c3<\/span> confidence level and are approximated in cases where these values have not been well established.<\/p>\n

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.<\/p>\n

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.<\/span><\/p>\n

Keywords<\/strong><\/p>\n

anisotropy; calibration; fluorescence; fluorometer; lifetime; limit of detection; luminescence; qualification; quantum yield; reference materials; spectral correction; spectrometer; spectroscopy; validation; verification; wavelength<\/p>\n

ICS Code<\/strong><\/p>\n

ICS Number Code 71.040.50 (Physicochemical methods of analysis)<\/p>\n

DOI:<\/strong> 10.1520\/E2719-09<\/p>\n<\/div>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
1<\/td>\nScope
Referenced Documents
Terminology <\/td>\n<\/tr>\n
3<\/td>\nSignificance and Use
General Guidelines
TABLE 1 <\/td>\n<\/tr>\n
4<\/td>\nWavelength Accuracy
TABLE 2 <\/td>\n<\/tr>\n
5<\/td>\nFIG. 1
FIG. 2 <\/td>\n<\/tr>\n
6<\/td>\nFIG. 3
FIG. 4 <\/td>\n<\/tr>\n
7<\/td>\nFIG. 5
FIG. 6 <\/td>\n<\/tr>\n
8<\/td>\nSpectral Slit Width Accuracy
Linearity of the Detection System
FIG. 7 <\/td>\n<\/tr>\n
9<\/td>\nSpectral Correction of Detection System Responsivity
FIG. 8
TABLE 3 <\/td>\n<\/tr>\n
10<\/td>\nSpectral Correction of Excitation Beam Intensity
FIG. 9
TABLE 4 <\/td>\n<\/tr>\n
11<\/td>\nFIG. 10 <\/td>\n<\/tr>\n
12<\/td>\nCalibration Curves for Concentration <\/td>\n<\/tr>\n
13<\/td>\nDay-to-Day and Instrument-to-Instrument Intensity
Limit of Detection and Sensitivity (
) (Test Method
)
Lifetimes (
,
) <\/td>\n<\/tr>\n
14<\/td>\nFluorescence Quantum Yield (
) <\/td>\n<\/tr>\n
15<\/td>\nFluorescence Anisotropy (
)
Keywords
REFERENCES <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

E2719-09 Standard Guide for Fluorescence—Instrument Calibration and Qualification<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
ASTM<\/b><\/a><\/td>\n2009<\/td>\n17<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":14637,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[1001,2637],"product_tag":[],"class_list":{"0":"post-14636","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-71-040-50","7":"product_cat-astm","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/14636","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/14637"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=14636"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=14636"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=14636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}