{"id":363760,"date":"2024-10-20T01:47:20","date_gmt":"2024-10-20T01:47:20","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-iso-ts-234592021\/"},"modified":"2024-10-26T02:48:46","modified_gmt":"2024-10-26T02:48:46","slug":"bsi-pd-iso-ts-234592021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-iso-ts-234592021\/","title":{"rendered":"BSI PD ISO\/TS 23459:2021"},"content":{"rendered":"

This document specifies measurement protocols and test conditions to determine alterations to protein secondary structure induced by their interaction with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy.<\/p>\n

This document does not apply to the characterization of conformational changes of disordered proteins.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Abbreviated terms
5 Nanomaterial protein interactions <\/td>\n<\/tr>\n
11<\/td>\n6 Sample preparation
6.1 General
6.2 Desired properties of the UV-CD quartz cell
6.3 Preparation of protein solution <\/td>\n<\/tr>\n
12<\/td>\n6.4 Instrumental setting condition
6.5 Recording UV-CD spectra procedure
6.5.1 General
6.5.2 Buffer
6.5.3 Protein sample <\/td>\n<\/tr>\n
13<\/td>\n6.5.4 Stability of NP suspension in the protein solution
6.6 Preparation of protein-NPs conjugated suspension
6.7 UV-CD spectra measurement <\/td>\n<\/tr>\n
14<\/td>\n6.8 Calculation of molar ellipticity
6.9 Data analysis <\/td>\n<\/tr>\n
15<\/td>\n7 Test report <\/td>\n<\/tr>\n
16<\/td>\nAnnex A (informative) Typical UV-CD spectra of proteins <\/td>\n<\/tr>\n
17<\/td>\nAnnex B (informative) Literature survey on structural changes of NOAA and proteins <\/td>\n<\/tr>\n
23<\/td>\nAnnex C (informative) Description of buffers that can be used for protein solubility <\/td>\n<\/tr>\n
27<\/td>\nAnnex D (informative) Unit conversions in CD measurements <\/td>\n<\/tr>\n
28<\/td>\nAnnex E (informative) Calculating the concentration range of the sample <\/td>\n<\/tr>\n
29<\/td>\nAnnex F (informative) Methods for estimation of secondary structures of protein <\/td>\n<\/tr>\n
30<\/td>\nAnnex G (informative) Typical data of UV-CD used for estimation of secondary structures of protein <\/td>\n<\/tr>\n
31<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Nanotechnologies. Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n36<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":363769,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-363760","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/363760","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/363769"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=363760"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=363760"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=363760"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}