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BS ISO 7358:2021

BS ISO 7358:2021

$102.76

Essential oils of bergamot, lemon, bitter orange and lime, fully or partially reduced in bergapten. Determination of bergapten content by high-performance liquid chromatography (HPLC)

Published By Publication Date Number of Pages
BSI 2021 18
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This document specifies a high-performance liquid chromatographic (HPLC) method, using either an internal standard or external standard, for the determination of the bergapten content in essential oil of bergamot [Citrus aurantium ssp. bergamia (Risso et Poit.) Wight et Arn. ex Engl.], in essential oil of lemon [Citrus limon (L.) Burm. f.], in essential oil of bitter orange (Citrus bigaradia Risso) and in essential oil of lime [Citrus aurantiifolia (Christm.) Swingle and Citrus latifolia Tanaka], all of them fully or partially reduced in bergapten.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
8 6 Apparatus
6.1 Common laboratory equipment
9 6.2 HPLC system
7 Sample preparation
8 Procedure
8.1 Operating conditions
8.2 Determination
8.2.1 HPLC in normal phase
11 8.2.2 HPLC in reversed phase
12 9 Calculation — Normal phase or reversed phase HPLC
9.1 Internal standard method
13 9.2 External standard method
10 Precision — Repeatability
11 Test report
15 Annex A (informative) Typical chromatogram of the reference substances using high-performance liquid chromatography (HPLC) of reference compounds in reversed phase
16 Annex B (informative) Bergapten UV-Vis spectrum
17 Bibliography

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BS ISO 7358:2021
$102.76