{"id":338472,"date":"2024-10-19T23:41:07","date_gmt":"2024-10-19T23:41:07","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-172522020\/"},"modified":"2024-10-25T22:47:15","modified_gmt":"2024-10-25T22:47:15","slug":"bs-en-172522020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-172522020\/","title":{"rendered":"BS EN 17252:2020"},"content":{"rendered":"
This document specifies a procedure for the determination of phomopsin A in lupin seeds and lupin-derived products based on liquid chromatography with tandem mass spectrometry (LC-MS\/MS). Several phomopsins exist, i.e. phomopsin A, B, C and D, but the method only deals with the quantitative measurement of phomopsin A due to lack of commercially available analytical reference standards for the other phomopsins.<\/p>\n
The method has been validated for phomopsin A in naturally contaminated lupin seeds, lupin flour and crisp bread at levels ranging from approximately 5 \u00b5g\/kg to 60 \u00b5g\/kg.<\/p>\n
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 6 Apparatus and equipment <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 7 Procedure 7.1 Preparation of the test sample 7.2 Extraction 7.3 Preparation of sample test solutions 7.4 LC-MS\/MS analysis 7.4.1 General <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 7.4.2 Batch composition and analytical sequence 7.4.3 Identification 7.4.4 Calibration <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 8 Calculation 9 Precision 9.1 General 9.2 Repeatability 9.3 Reproducibility <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 10 Test report <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | Annex A (informative)Precision data <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | Annex B (informative)Examples conditions for suitable LC-MS\/MS systems with typical chromatograms <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Foodstuffs. Determination of phomopsin A in lupin seeds and lupin derived products by HPLC-MS\/MS<\/b><\/p>\n |