{"id":341001,"date":"2024-10-19T23:54:03","date_gmt":"2024-10-19T23:54:03","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-iso-ts-188672015\/"},"modified":"2024-10-25T23:05:11","modified_gmt":"2024-10-25T23:05:11","slug":"bsi-pd-cen-iso-ts-188672015","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-iso-ts-188672015\/","title":{"rendered":"BSI PD CEN ISO\/TS 18867:2015"},"content":{"rendered":"

This Technical Specification specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica<\/i> and one for detection of Y. pseudotuberculosis<\/i> by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis<\/i>, the causative agent of bubonic and pneumonic plague harbours a variant of the ail<\/i> gene as well and will be detected by the same primer\/probe set as Y. pseudotuberculosis<\/i>. However, Y. pestis<\/i> is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nContents Page <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
8<\/td>\nForeword <\/td>\n<\/tr>\n
9<\/td>\nIntroduction <\/td>\n<\/tr>\n
10<\/td>\n1\tScope
2\tNormative references
3\tTerms and definitions
4\tPrinciples
4.1\tGeneral <\/td>\n<\/tr>\n
11<\/td>\n4.2\tMicrobial enrichment
4.3\tNucleic acid extraction
4.4\tAmplification and detection
4.5\tIsolation
5\tReagents
5.1\tGeneral
5.2\tCulture media
5.2.1\tGeneral
5.2.2\tDiluent
5.2.3\tEnrichment media <\/td>\n<\/tr>\n
13<\/td>\n5.2.4\tSelective solid medium <\/td>\n<\/tr>\n
14<\/td>\n5.2.5\tPotassium hydroxide in saline solution, KOH
5.3\tNucleic acid extraction
5.4\tReagents for PCR
5.5\tPrimers and probes
6\tApparatus and equipment
6.1\tGeneral <\/td>\n<\/tr>\n
15<\/td>\n6.2\tEquipment for sample preparation prior to enrichment
6.3\tEquipment for microbial enrichment
6.4\tEquipment for nucleic acid extraction
6.5\tEquipment for real-time PCR
7\tSampling
8\tProcedure
8.1\tSample preparation prior to enrichment
8.1.1\tGeneral
8.1.2\tPreparation of the sample <\/td>\n<\/tr>\n
16<\/td>\n8.2\tMicrobial enrichment
8.2.1\tPathogenic Y. enterocolitica
8.2.2\tY. pseudotuberculosis
8.2.3\tPathogenic Y. enterocolitica and Y. pseudotuberculosis
8.3\tIsolation of colonies, optional
8.3.1\tPathogenic Y. enterocolitica
8.3.2\tY. pseudotuberculosis <\/td>\n<\/tr>\n
17<\/td>\n8.3.3\tProcess controls
8.4\tNucleic acid extraction
8.5\tPCR amplification
8.5.1\tGeneral
8.5.2\tPCR controls
8.6\tConfirmation of the PCR product
8.6.1\tGeneral
8.6.2\tInterpretation of the PCR result <\/td>\n<\/tr>\n
18<\/td>\n9\tTest report <\/td>\n<\/tr>\n
19<\/td>\nAnnex\u00a0A (normative) PCR detection and isolation of pathogenic Y. enterocolitica (see Figure\u00a0A.1) <\/td>\n<\/tr>\n
20<\/td>\nAnnex\u00a0B (informative) Real-time PCR for detection of Y. enterocolitica <\/td>\n<\/tr>\n
30<\/td>\nAnnex\u00a0C (informative) Detection and isolation of Y. pseudotuberculosis <\/td>\n<\/tr>\n
35<\/td>\nAnnex\u00a0D (informative) Simultaneous detection of pathogenic Y. enterocolitica and Y.\u00a0pseudotuberculosis using multiplex real-time PCR <\/td>\n<\/tr>\n
38<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2016<\/td>\n42<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":341009,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-341001","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/341001","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/341009"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=341001"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=341001"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=341001"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}