{"id":402607,"date":"2024-10-20T05:02:00","date_gmt":"2024-10-20T05:02:00","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-177172022\/"},"modified":"2024-10-26T08:57:43","modified_gmt":"2024-10-26T08:57:43","slug":"bsi-pd-cen-ts-177172022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-177172022\/","title":{"rendered":"BSI PD CEN\/TS 17717:2022"},"content":{"rendered":"<p>This document describes a method for the detection of Salmonella spp. in biostimulants of the following Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products, as described in the Regulation (EU) 2019\/1009 of the European Parliament and of the Council [1]: \u2014 PFC 6(A): Microbial plant biostimulant; \u2014 PFC 6(B): Non-microbial plant biostimulant; \u2014 CMC 7: Microorganisms. It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).<\/p>\n<h4>PDF Catalog<\/h4>\n<table border=\"1px\" class=\"des-table\">\n<tr>\n<th>PDF Pages<\/th>\n<th>PDF Title<\/th>\n<\/tr>\n<tr>\n<td><b>2<b><\/td>\n<td>undefined  <\/td>\n<\/tr>\n<tr>\n<td><b>7<b><\/td>\n<td>1 Scope <br \/> 2 Normative references <br \/> 3 Terms and definitions  <\/td>\n<\/tr>\n<tr>\n<td><b>8<b><\/td>\n<td>4 Principle <br \/> 4.1  General <br \/> 4.2  Enrichment in selective liquid medium <br \/> 4.3  Plating out on selective solid media <br \/> 4.4  Confirmation <br \/> 5 Culture media, reagents, antisera <br \/> 5.1 General  <\/td>\n<\/tr>\n<tr>\n<td><b>9<b><\/td>\n<td>5.2 Isolation chromogenic agar <br \/> 5.3 Non-selective agar <br \/> 5.4 Confirmation selective agar <br \/> 6 Equipment and consumables  <\/td>\n<\/tr>\n<tr>\n<td><b>10<b><\/td>\n<td>7 Sampling <br \/> 8 Preparation of test sample <br \/> 9 Procedure <br \/> 9.1 Test portion and initial suspension  <\/td>\n<\/tr>\n<tr>\n<td><b>11<b><\/td>\n<td>9.2 Selective enrichment <br \/> 9.3 Isolation <br \/> 9.4 Confirmation <br \/> 9.4.1 General <br \/> 9.4.2 Selection of colonies for confirmation <br \/> 9.4.3 Serological testing <br \/> 9.4.3.1 General  <\/td>\n<\/tr>\n<tr>\n<td><b>12<b><\/td>\n<td>9.4.3.2 Elimination of auto-agglutinable strains <br \/> 9.4.3.3 Examination for O-antigens <br \/> 9.4.3.4 Examination for H-antigens <br \/> 9.4.3.5 Interpretation of serological reactions  <\/td>\n<\/tr>\n<tr>\n<td><b>13<b><\/td>\n<td>9.4.4 Selective medium for H2S production <br \/> 9.4.5 Serotyping <br \/> 10 Expression of results <br \/> 11 Performance characteristics of the method <br \/> 11.1 Interlaboratory studies  <\/td>\n<\/tr>\n<tr>\n<td><b>14<b><\/td>\n<td>11.2 Sensitivity <br \/> 11.3 Specificity <br \/> 11.4 LOD50 <br \/> 12  Test report  <\/td>\n<\/tr>\n<tr>\n<td><b>15<b><\/td>\n<td>Annex A (normative)Diagram of the procedures  <\/td>\n<\/tr>\n<tr>\n<td><b>16<b><\/td>\n<td>Annex B (normative)Culture media and reagents <br \/> B.1 General <br \/> B.2 Buffered peptone water (BPW) <br \/> B.2.1 Composition <br \/> B.2.2 Preparation  <\/td>\n<\/tr>\n<tr>\n<td><b>17<b><\/td>\n<td>B.3 Novobiocin solution <br \/> B.3.1 Composition <br \/> B.3.2 Preparation <br \/> B.4 Nutrient agar (example of non-selective medium) <br \/> B.4.1 Composition <br \/> B.4.2 Preparation <br \/> B.4.3 Preparation of nutrient agar plates  <\/td>\n<\/tr>\n<tr>\n<td><b>18<b><\/td>\n<td>B.5 Triple sugar iron agar (TSI agar ; example for H2S production agar) <br \/> B.5.1 Composition <br \/> B.5.2 Preparation <br \/> B.6 Saline solution <br \/> B.6.1 Composition <br \/> B.6.2 Preparation  <\/td>\n<\/tr>\n<tr>\n<td><b>19<b><\/td>\n<td>B.7 Antisera <br \/> B.8 Performance testing for the quality assurance of the culture media  <\/td>\n<\/tr>\n<tr>\n<td><b>20<b><\/td>\n<td>Annex C (informative)Examples of selective plating-out media  <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p><b>Plant biostimulants. Detection of Salmonella spp.<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2022<\/td>\n<td>22<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":402616,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-402607","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/402607","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/402616"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=402607"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=402607"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=402607"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}