{"id":360040,"date":"2024-10-20T01:28:58","date_gmt":"2024-10-20T01:28:58","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-181842019-tc\/"},"modified":"2024-10-26T02:13:45","modified_gmt":"2024-10-26T02:13:45","slug":"bs-iso-181842019-tc","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-181842019-tc\/","title":{"rendered":"BS ISO 18184:2019 – TC"},"content":{"rendered":"

This document specifies testing methods for the determination of the antiviral activity of the textile products against specified viruses. Due to the individual sensitivities, the results of one test virus cannot be transposed to other viruses.<\/p>\n

The textile products include woven and knitted fabrics, fibres, yarns, braids, etc.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
3<\/td>\nNational foreword
Compliance with a British Standard cannot confer immunity from legal obligations.
Amendments\/corrigenda issued since publication <\/td>\n<\/tr>\n
4<\/td>\nTextiles \u2014 Determination of antiviral
activity of textile products <\/td>\n<\/tr>\n
5<\/td>\nCOPYRIGHT PROTECTED DOCUMENT <\/td>\n<\/tr>\n
8<\/td>\nForeword <\/td>\n<\/tr>\n
9<\/td>\nIntroduction <\/td>\n<\/tr>\n
10<\/td>\nTextiles \u2014 Determination of antiviral activity of textile products
1 Scope
2 Normative references
3 Terms and definitions
3.1 <\/td>\n<\/tr>\n
11<\/td>\n3.2
3.3
3.4
3.5
reference cloth control fabric
3.6
3.7
3.8
3.9 <\/td>\n<\/tr>\n
12<\/td>\n3.10
3.11
3.12
3.13
3.14
4 Principle
5 Virus and host cell
6 Warning <\/td>\n<\/tr>\n
13<\/td>\n7 Apparatus
7.6 Washing machine. <\/td>\n<\/tr>\n
14<\/td>\nFigure 1 \u2014 96 wells microplate for TCID50 method
Figure 2 \u2014 6 wells plastic plate for plaque assay <\/td>\n<\/tr>\n
15<\/td>\nFigure 3 \u2014 Flask for cell culture use
7.26 Centrifuge tube.
8 Sterilization of apparatus <\/td>\n<\/tr>\n
16<\/td>\n9 Reagent and medium
9.3 7,5 % sodium bicarbonate solution.
9.4 Formalin solution.
9.5 Methylene blue solution, use for the cells dyeing.
9.4 Formaldehyde solution.
9.5 Methylene blue solution. <\/td>\n<\/tr>\n
17<\/td>\n9.6 Inactivated fetal bovine serum (FBS)
9.7 Growth medium, used for cell culture.
9.8 Maintenance medium, used for cell culture. <\/td>\n<\/tr>\n
18<\/td>\n9.9 Double concentration of the maintenance medium (9.8).
9.10 0,01 mol\/l phosphate buffered saline PBS (-).
9.11 Trypsin derived from beef pancreas and PBS (-) solution. <\/td>\n<\/tr>\n
19<\/td>\n9.12 Trypsin EDTA solution.
9.13 DEAE-dextran solution.
9.14 Agar medium, used for the plaque assay. preparation. <\/td>\n<\/tr>\n
20<\/td>\n9.14.1 A liquid.Liquid A.
9.14.2 Liquid B liquid. <\/td>\n<\/tr>\n
21<\/td>\n9.16 Maintenance medium, used for cell culture and for TCID50 method.Maintenance mediumwith Trypsin.
10 Preparation
10.1 Restoration of host cell from cryopreservation <\/td>\n<\/tr>\n
22<\/td>\n10.2 Subculture of host cell
10.3 Cell culture for the infectious virus titre assay <\/td>\n<\/tr>\n
23<\/td>\n10.4 Preparation for test virus
10.4.1 General
10.4.2 Influenza virus <\/td>\n<\/tr>\n
24<\/td>\n10.4.3 Feline calicivirus <\/td>\n<\/tr>\n
25<\/td>\n10.4.4 Infectivity titre of the test viruses
10.4.4.1 Preparation for series of the dilution for the virus suspension
10.4.4.12 Infectivity measurement
10.4.4.2 Determination of the infectious titre
10.5 Preparation for test specimen
10.5.1 Control fabric <\/td>\n<\/tr>\n
26<\/td>\n10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test
10.6.1 General
10.6.2 Verification of cytotoxic effect
10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity <\/td>\n<\/tr>\n
27<\/td>\n10.6.3 Verification of cell sensitivity to virus and the inactivation of antiviral activity
11 Test procedure
11.1 Preparation of specimen
11.2 Inoculation of virus to the specimens
11.2 Deposit of virus to the specimens
11.3 Contacting time <\/td>\n<\/tr>\n
28<\/td>\n11.4 Wash-out of virus immediately after inoculationdeposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension <\/td>\n<\/tr>\n
29<\/td>\n13 Infective titre measurement
13 Determination of the infectious titre
13.1 Plaque assay
14 Calculation of infectivity titre
14.2.1 The Behrens and Karber method <\/td>\n<\/tr>\n
30<\/td>\n14.2.2 Example of calculation <\/td>\n<\/tr>\n
31<\/td>\nFigure 4 \u2014 Example of TCID50 method
14.3 Test result
14.3.1 Verification of this test <\/td>\n<\/tr>\n
32<\/td>\n14.3.2 Calculation of antiviral activity value
15 Test report <\/td>\n<\/tr>\n
33<\/td>\n(normativeinformative)
Table A.1 \u2014 Viruses, host cells and media used for this standarddocument <\/td>\n<\/tr>\n
34<\/td>\nAnnex B
(normative)
B.1 Test procedure <\/td>\n<\/tr>\n
35<\/td>\nB.2 Determination of PFU
B.2.1 General
Table B.2 \u2014 Interpretation of the data <\/td>\n<\/tr>\n
37<\/td>\nAnnex C
(normative)
C.1 Test procedure
C.1.1 Pick up 96 wells microplate with the monolayer cells grown in the each well of Clause 10.3 and observe by a microscope of a confluent state of the grown cells. After confirmation, drain extra growth medium from the plate.Observe at the microscop… <\/td>\n<\/tr>\n
38<\/td>\nD.2 Composition of EMEM
Table D.1 \u2014 Composition of EMEM <\/td>\n<\/tr>\n
39<\/td>\nTable D.2 \u2014 The Composition of RPMI 1640 <\/td>\n<\/tr>\n
40<\/td>\nAnnex E
(informative)
E.1 General
Table E.1 \u2014 Polio virus strain and host cell <\/td>\n<\/tr>\n
41<\/td>\nF.1E.1 General
F.2 Scope
E.2 Overview
F.3.1 SPF embryonated hen’s eggs
E.3 Specific pathogen free (SPF) embryonated hen’s eggs
F.4E.4 Preparation of specimen
F.5E.5 Preparation of the embryonated hen’s eggs
F.6E.6 Preparation of virus <\/td>\n<\/tr>\n
42<\/td>\nF.6.1E.6.1 Virus cultivation
F.6.2E.6.2 Preparation of 0,5 % chicken red blood cell suspension
F.7E.7 Test procedure
F.7.1E.7.1 General <\/td>\n<\/tr>\n
43<\/td>\nFigure F.1Figure E.1\u2014 General process image of embryonated eggs method
F.7.3E.7.3 Inoculation of the reacted virus suspension to 10 day old embryonated hen’s eggs
F.7.4E.7.4 Virus assay
F.7.5E.7.5 Calculation of the virus titre <\/td>\n<\/tr>\n
44<\/td>\nTable F.1E.1 \u2014 Example of the EID test <\/td>\n<\/tr>\n
46<\/td>\nAnnex F
(informative)
Tabelle GTable F.1 \u2014 Antiviral performance standard <\/td>\n<\/tr>\n
47<\/td>\nTable H.1 \u2014 Sample details for Round robin test
Table G.1 \u2014 Sample details for interlaboratory test
H.3G.3 Test condition
H.4G.4 Test result
H.4.1G.4.1 Test result of a testing house <\/td>\n<\/tr>\n
48<\/td>\nTable H.2G.2\u2014 Test result of a testing house
H.4.2G.4.2 Test result of a laboratory
Table H.3G.3\u2014 Test result of a laboratory <\/td>\n<\/tr>\n
49<\/td>\nAnnex IH
(informative)
I.1H.1 Participants
I.2H.2 Sample
Table I.1H.1\u2014 Sample details for Round robininterlaboratory test <\/td>\n<\/tr>\n
50<\/td>\nI.4H.4 Test result
I.4.1H.4.1 Sample A
Table I.2Table H.2\u2014 Round robinInterlaboratory test result for sample A
Table I.3H.3\u2014 Round robinInterlaboratory test result for sample B <\/td>\n<\/tr>\n
51<\/td>\nI.4.3H.4.3 Sample C
Table I.4H.4 \u2014 Round robinInterlaboratory test result for sample C
Table I.5H.5\u2014 Summary of Antiviralantiviral activity <\/td>\n<\/tr>\n
52<\/td>\nBibliography <\/td>\n<\/tr>\n
54<\/td>\nNational foreword <\/td>\n<\/tr>\n
59<\/td>\nForeword <\/td>\n<\/tr>\n
60<\/td>\nIntroduction <\/td>\n<\/tr>\n
61<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
63<\/td>\n4 Principle
5 Virus and host cell
6 Warning
7 Apparatus <\/td>\n<\/tr>\n
66<\/td>\n8 Sterilization of apparatus
9 Reagent and medium <\/td>\n<\/tr>\n
67<\/td>\n9.4 Formaldehyde solution.
9.6 Inactivated fetal bovine serum (FBS) <\/td>\n<\/tr>\n
71<\/td>\n10 Preparation
10.1 Restoration of host cell from cryopreservation <\/td>\n<\/tr>\n
72<\/td>\n10.2 Subculture of host cell <\/td>\n<\/tr>\n
73<\/td>\n10.3 Cell culture for the infectious virus titre assay
10.4 Preparation for test virus
10.4.1 General
10.4.2 Influenza virus <\/td>\n<\/tr>\n
74<\/td>\n10.4.3 Feline calicivirus <\/td>\n<\/tr>\n
75<\/td>\n10.4.4 Infectivity titre of the test viruses <\/td>\n<\/tr>\n
76<\/td>\n10.5 Preparation for test specimen
10.5.1 Control fabric
10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test
10.6.1 General <\/td>\n<\/tr>\n
77<\/td>\n10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity
11 Test procedure
11.1 Preparation of specimen
11.2 Deposit of virus to the specimens
11.3 Contacting time <\/td>\n<\/tr>\n
78<\/td>\n11.4 Wash-out of virus immediately after deposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension
13 Determination of the infectious titre
13.1 Plaque assay <\/td>\n<\/tr>\n
79<\/td>\n13.2 TCID50 method
14 Calculation of infectivity titre
14.1 Plaque assay
14.2 TCID50 method
14.2.1 Behrens and Karber method <\/td>\n<\/tr>\n
80<\/td>\n14.2.2 Example of calculation <\/td>\n<\/tr>\n
81<\/td>\n14.3 Test result
14.3.1 Verification of this test <\/td>\n<\/tr>\n
82<\/td>\n14.3.2 Calculation of antiviral activity value
15 Test report <\/td>\n<\/tr>\n
83<\/td>\nAnnex A (informative) Virus strains and host cells <\/td>\n<\/tr>\n
84<\/td>\nAnnex B (normative) Infectivity titre test: Plaque assay <\/td>\n<\/tr>\n
86<\/td>\nAnnex C (normative) Infectivity titre test: TCID50 method <\/td>\n<\/tr>\n
87<\/td>\nAnnex D (informative) Composition of media <\/td>\n<\/tr>\n
90<\/td>\nAnnex E (informative) Testing method using specific pathogen free (SPF) embryonated hen’s eggs <\/td>\n<\/tr>\n
95<\/td>\nAnnex F (informative) Antiviral efficacy \u2014 Antiviral performance of the products <\/td>\n<\/tr>\n
96<\/td>\nAnnex G (informative) Interlaboratory test result (1) <\/td>\n<\/tr>\n
98<\/td>\nAnnex H (informative) Interlaboratory test result (2) <\/td>\n<\/tr>\n
101<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Tracked Changes. Textiles. Determination of antiviral activity of textile products<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2020<\/td>\n0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":360047,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[878,2641],"product_tag":[],"class_list":{"0":"post-360040","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-59-080-01","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/360040","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/360047"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=360040"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=360040"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=360040"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}