{"id":375408,"date":"2024-10-20T02:43:29","date_gmt":"2024-10-20T02:43:29","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-145012021\/"},"modified":"2024-10-26T04:46:00","modified_gmt":"2024-10-26T04:46:00","slug":"bs-en-iso-145012021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-145012021\/","title":{"rendered":"BS EN ISO 14501:2021"},"content":{"rendered":"

This document specifies a method for the determination of aflatoxin M1<\/sub> content in milk and milk powder. The lowest level of validation is 0,08 \u00b5g\/kg for whole milk powder, i.e. 0,008 \u00b5g\/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 ?g\/kg for milk powder and 0,005 ?g\/kg for liquid milk. The limit of quantification (LOQ) is 0,1 ?g\/kg for milk powder and 0,01 ?g\/kg for liquid milk.<\/p>\n

The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
4<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\nForewords <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Principle
5 Reagents
5.1 Immunoaffinity column. <\/td>\n<\/tr>\n
11<\/td>\n5.3 Nitrogen gas.
5.4 Aflatoxin M1 standard solutions. <\/td>\n<\/tr>\n
12<\/td>\n6 Apparatus <\/td>\n<\/tr>\n
13<\/td>\n7 Sampling
8 Procedure
8.1 General
8.2 Preparation of test samples
8.2.1 Milk
8.2.2 Milk powder
8.3 Immunoaffinity column preparation <\/td>\n<\/tr>\n
14<\/td>\n8.4 Test sample purification
8.5 High performance liquid chromatography (HPLC)
8.5.1 Pump setting
8.5.2 Chromatographic performance
8.5.3 Calibration curve of aflatoxin M1 <\/td>\n<\/tr>\n
15<\/td>\n8.5.4 Analysis of the purified extracts and injection scheme
9 Calculation and expression of results
9.1 Skimmed milk
9.1.1 Calculation
9.1.2 Expression of results
9.2 Skimmed milk powder
9.2.1 Calculation <\/td>\n<\/tr>\n
16<\/td>\n9.2.2 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report <\/td>\n<\/tr>\n
17<\/td>\nAnnex A (informative) Results of an interlaboratory trial <\/td>\n<\/tr>\n
18<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":375416,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[960,2641],"product_tag":[],"class_list":{"0":"post-375408","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-100-10","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/375408","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/375416"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=375408"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=375408"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=375408"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}